Real Time PCR Predel

Thermocycleur pour PCR PREDEL

Real Time PCR Predel in technology relies on two essential elements :

 A ready-to-use kit.
 A real Time PCR machine allows for procedural ans analytic simplicity.

1st phase: denaturisation
The weak connections which ensured the cohesion of the DNA double helix are broken into to give two single DNA strands.
2nd phase: hybridization (annealing)
The hybridization of DNA primers rests on the principle of the pairing of complementary bases.
3rd phase: elongation (extension of primers)
The primers hybridized to the DNA are used as a starting point for polymerization of the complementary DNA single chain of the template DNA by the polymerase.

At each cycle, the DNA quantity doubles in the tube. Thus on the basis of two initial DNA strands we obtain a million copies in twenty cycles.